ACADEMIC INFORMATION
Hemostasis
[Technology]
(Basic Principle) The proteolytic mechanism of serine proteases?
관리자(bioms@hanmail.net)
2020.07.22조회 1425 |
|
The proteolytic mechanism of serine proteases
The proteolytic mechanism of serine proteases
The proteolytic action of a serine protease on its substrate comprises several steps starting with the formation of a non-covalent complex between the enzyme and the substrate. A nucleophilic attack by the serine hydroxyl group on the amide carbonyl carbon atom in the substrate results in cleavage of the amide bond and the formation of an acyl-enzyme intermediate.
The acyl-enzyme ester bond is then hydrolysed in the rate limiting step and the enzyme is now free to catalyze the cleavage of another substrate molecule.
|